Bacillus subtilis grown in a "breathing" vessel without sparger aeration.
Ken-Ichi YoshidaKen-Ichi YoshidaAyşegül ÖktemShu IshikawaJan Maarten van DijlMasato YotsuyaRyosuke SatoPublished in: Bioscience, biotechnology, and biochemistry (2024)
Here we present a "breathing" vessel consisting of expanded polytetrafluoroethylene, which allows gas exchange but no liquid permeation. The bacterial culture inside needs only agitation to promote air supply. Using this setup, a Bacillus subtilis cell factory for scyllo-inositol production grew to produce scyllo-inositol efficiently. The results indicate that our approach represents a sustainable "greener" approach for the cell factory.