Development and Validation of an LC-MS/MS Method for Simultaneous Determination of Short Peptide-Based Drugs in Human Blood Plasma.
Elizaveta N FisherEvgeny S MelnikovVladimir I GegechkoriNatalya V PotoldykovaDmitry V EnikeevKirill A PavlenkoSnezana Agatonovic-KustrinDavid William MortonGalina V RamenskayaPublished in: Molecules (Basel, Switzerland) (2022)
A novel HPLC-ESI-MS/MS method for simultaneous gonadotropin-releasing hormone (GnRH) analogs and somatostatin analog quantitation was developed and validated. The developed method was successfully applied to pharmacokinetic studies. The sample preparation process included solid-phase extraction (SPE). Effective chromatographic separation of the analytes and internal standard (dalargin) was achieved with a C18 column, using a gradient elution with two mobile phases: 0.1% v / v formic acid (aqueous solution) and 0.1% v / v formic acid (acetonitrile solution). The linearity of the method was demonstrated within a concentration range of 0.5-20 ng/mL, with correlation coefficients between 0.998-0.999 for goserelin, buserelin, triptorelin, and octreotide, respectively. The relative standard deviation (RSD, %) values for method accuracy and precision did not exceed 20% at the lower level of quantitation (LLOQ) or 15% at other concentration levels.
Keyphrases
- solid phase extraction
- simultaneous determination
- liquid chromatography tandem mass spectrometry
- liquid chromatography
- molecularly imprinted
- high performance liquid chromatography
- tandem mass spectrometry
- ms ms
- ultra high performance liquid chromatography
- gas chromatography mass spectrometry
- mass spectrometry
- aqueous solution
- high resolution mass spectrometry
- gas chromatography
- endothelial cells
- neuroendocrine tumors
- molecular docking
- induced pluripotent stem cells