A multicenter study to evaluate automated platelet aggregometry on Sysmex CS-series coagulation analyzers-preliminary findings.

A standard panel of agonists can be used on the Sysmex CS-2x00 series analyzers: ADP (10, 5, 2.5, and 1.25 μM); 1 mM arachidonic acid; 1 μM U46619; 2.5 and 1.25 μg/mL collagen; 1.25 and 0.5 g/L ristocetin; 5 μM epinephrine; 5 and 10 μM TRAP; and saline. Aggregation should be observed for 600 seconds for all agonists except TRAP and U46619, which require 300 seconds. If further studies confirm these concentrations detect platelet disorders then Sysmex CS-series analyzers could replace dedicated aggregometers, or perform LTA where it is currently not available.