Login / Signup

A Temporary Immersion System Improves Regeneration of In Vitro Irradiated Recalcitrant Indica Rice ( Oryza sativa L.) Embryogenic Calli.

Alejandro Hernández SotoJason PérezRebeca Fait-ZúñigaRandall Rojas-VásquezAndrés Gatica-AriasWalter Vargas-SeguraAna Abdelnour-Esquivel
Published in: Plants (Basel, Switzerland) (2022)
The development of gamma ray-mutated rice lines is a solution for introducing genetic variability in indica rice varieties already being used by farmers. In vitro gamma ray ( 60 Co) mutagenesis reduces chimeras and allows for a faster selection of desirable traits but requires the optimization of the laboratory procedure. The objectives of the present work were sequencing of mat K and rbc L, the in vitro establishment of recalcitrant rice embryogenic calli, the determination of their sensitivity to gamma radiation, and optimization of the generation procedure. All sequenced genes matched perfectly with previously reported mat K and rbc L O. sativa genes. Embryogenic calli induction improved using MS medium containing 2 mg L -1 2,4-D, and regeneration was achieved with MS medium with 3 mg L -1 BA and 0.5 mg L -1 NAA. The optimized radiation condition was 60 Gy, (LD20 = 64 Gy) with 83% regeneration. An immersion system (RITA ® , Saint-Mathieu-de-Tréviers, France) of either 60 or 120 s every 8 h allowed systematic and homogeneous total regeneration of the recalcitrant line. Other well-known recalcitrant cultivars, CR1821 and CR1113, also had improved regeneration in the immersion system. To our knowledge, this is the first study reporting the use of an immersion system to allow for the regeneration of gamma-ray mutants from recalcitrant indica rice materials.
Keyphrases
  • stem cells
  • genome wide
  • wound healing
  • multiple sclerosis
  • mass spectrometry
  • healthcare
  • ms ms
  • minimally invasive
  • gene expression
  • red blood cell
  • radiation induced
  • copy number
  • drug induced
  • simultaneous determination