Login / Signup

Assessing the prospect of XAFS experiments of metalloproteins under in vivo conditions at Indus-2 synchrotron facility, India.

Debdutta LahiriRicha AgrawalKhileshwari ChandravanshiParasmani RajputAnkur AgrawalAshutosh DwivediRavindra D MakdeS N JhaNandini Garg
Published in: Journal of synchrotron radiation (2023)
The feasibility of X-ray absorption fine-structure (XAFS) experiments of ultra-dilute metalloproteins under in vivo conditions (T = 300 K, pH = 7) at the BL-9 bending-magnet beamline (Indus-2) is reported, using as an example analogous synthetic Zn (0.1 mM) M1dr solution. The (Zn K-edge) XAFS of M1dr solution was measured with a four-element silicon drift detector. The first-shell fit was tested and found to be robust against statistical noise, generating reliable nearest-neighbor bond results. The results are found to be invariant between physiological and non-physiological conditions, which confirms the robust coordination chemistry of Zn with important biological implications. The scope of improving spectral quality for accommodation of higher-shell analysis is addressed.
Keyphrases
  • heavy metals
  • air pollution
  • high resolution
  • editorial comment
  • dual energy
  • computed tomography
  • drug discovery
  • image quality
  • transition metal