Tracking Plasma Membrane Damage Using a Ruthenium(II) Complex Phosphorescent Indicator Paired with Cholesterol.
Qianqian FaXiaona GaoWenzhu ZhangJunyu RenBo SongJingli YuanPublished in: Inorganic chemistry (2024)
Long-term in situ plasma membrane-targeted imaging is highly significant for investigating specific biological processes and functions, especially for the imaging and tracking of apoptosis processes of cells. However, currently developed membrane probes are rarely utilized to monitor the in situ damage of the plasma membrane. Herein, a transition-metal complex phosphorescent indicator, Ru-Chol , effectively paired with cholesterol, exhibits excellent properties on staining the plasma membrane, with excellent antipermeability, good photostability, large Stokes shift, and long luminescence lifetime. In addition, Ru-Chol not only has the potential to differentiate cancerous cells from normal cells but also tracks in real time the entire progression of cisplatin-induced plasma membrane damage and cell apoptosis. Therefore, Ru-Chol can serve as an efficient tool for the monitoring of morphological and physiological changes in the plasma membrane, providing assistance for drug screening and early diagnosis and treatment of diseases, such as immunodeficiency, diabetes, cirrhosis, and tumors.
Keyphrases
- cell cycle arrest
- induced apoptosis
- oxidative stress
- endoplasmic reticulum stress
- high resolution
- cell death
- energy transfer
- transition metal
- type diabetes
- cardiovascular disease
- pi k akt
- fluorescence imaging
- signaling pathway
- small molecule
- mass spectrometry
- metabolic syndrome
- emergency department
- risk assessment
- cancer therapy
- single molecule
- human health
- drug delivery
- skeletal muscle