The Cdc25/Ras/cAMP-dependent protein kinase A signaling pathway regulates proline utilization in wine yeast Saccharomyces cerevisiae under a wine fermentation model.
Akira NishimuraKazuki IchikawaHayate NakazawaRyoya TanahashiFumika MoritaIrnayuli SitepuKyria Boundy-MillsGlen FoxHiroshi TakagiPublished in: Bioscience, biotechnology, and biochemistry (2022)
Proline is a predominant amino acid in grape must, but it is poorly utilized by the yeast Saccharomyces cerevisiae in wine-making processes. This sometimes leads to a nitrogen deficiency during fermentation and proline accumulation in wine. In this study, we clarified that a glucose response is involved in an inhibitory mechanism of proline utilization in yeast. Our genetic screen showed that strains with a loss-of-function mutation on the CDC25 gene can utilize proline even under fermentation conditions. Cdc25 is a regulator of the glucose response consisting of the Ras/cAMP-dependent protein kinase A (PKA) pathway. Moreover, we found that activation of the Ras/PKA pathway is necessary for the inhibitory mechanism of proline utilization. The present data revealed that crosstalk exists between the carbon and proline metabolisms. Our study could hold promise for the development of wine yeast strains that can efficiently assimilate proline during the fermentation processes.
Keyphrases
- saccharomyces cerevisiae
- protein kinase
- signaling pathway
- escherichia coli
- genome wide
- cell cycle
- amino acid
- blood glucose
- epithelial mesenchymal transition
- transcription factor
- type diabetes
- gene expression
- electronic health record
- oxidative stress
- cell proliferation
- blood pressure
- metabolic syndrome
- artificial intelligence
- deep learning
- data analysis
- endoplasmic reticulum stress