Unveiling the Impact of Gelation Temperature on the Rheological and Microstructural Properties of Type A Gelatin Hydrogels.

Gelatin-based hydrogels have garnered significant attention in the fields of drug delivery systems and tissue engineering owing to their biodegradability, biocompatibility, elasticity, flexibility and nontoxic nature. However, there is a lack of information regarding type-A-gelatin-based hydrogels. In this sense, the main aim of this work was the evaluation of the properties of type-A-gelatin-based hydrogel achieved using two different gelation temperatures (4 °C and 20 °C). Thus, the main novelty of this study lies in the analysis of the impact of gelation time on the rheological and microstructural properties of type-A-gelatin-based hydrogels. Moreover, the addition of a drug was also analyzed in order to evaluate the hydrogels' behavior as a drug delivery system. For this purpose, rheological (strain, frequency sweep tests and flow curves) and microstructural (SEM) characterizations were carried out. The results demonstrated that lowering the gelation temperature improved the rheological properties of the systems, obtaining hydrogels with an elastic modulus of 20 kPa when processing at 4 °C. On the other hand, the increase in the gelation temperature improved the critical strain of the systems at low temperatures. In conclusion, this work showed the feasibility of producing hydrogels with potential application in drug delivery with different properties, varying the testing temperature and incorporating tetracycline into their formulation.