Specific monitoring of aflatoxin M1 in real samples using aptamer binding to DNFS based on turn-on method: A novel biosensor.

In the present work, a novel biocompatible scaffold was fabricated for the DNA aptamer immobilization. For the first time, amino-functionalized dendritic fibrous nanosilica (KCC-1-nPr-NH2 ) and gold nanoparticle supported by chitosan (AuNPs-CS) were synthesized and electrodeposited successfully on the surface of the glassy carbon electrode by chronoamperometry technique. Unique oligonucleotide of aflatoxin M1 (5'-ATC CGT CAC ACC TGC TCT GAC GCT GGG GTC GAC CCG GAG AAA TGC ATT CCC CTG TGG TGT TGG CTC CCG TAT) labeled by toluidine blue was immobilization on the prepared interface. Hence, a novel aptamer-based bioassay was formed for highly sensitive quantitation of AFM1 using cyclic voltammetry and differential plus voltammetry. The structure and morphology of GQDs-CS/KCC-1-nPr-NH2 were investigated by Fourier-transform infrared spectroscopy, X-ray diffraction, atomic force, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The achieved low limit of quantification of apta-assay for detection of AFM1 was 10fM. Also, calibration curve was linear from 0.1μM to 10fM in real samples. The proposed apta-assay has acceptable long-term stability. Designed aptasensor has a lot of remarkable advantages including excellent selectivity, sensitivity, and stability that could be used as facile bio-device for the determination of AFM1 in milk samples.