Quantifying mitochondrial DNA copy number using robust regression to interpret real time PCR results.
Paulo RefinettiDavid WarrenStephan MorgenthalerPer O EkstrømPublished in: BMC research notes (2017)
Comparing the two methods when analysing 96 identical PCR preparations showed similar distributions of the estimated copy numbers. Estimating the efficiency with the [Formula: see text] method, however, required a dilution series, which is not necessary for the robust regression method. We used rtPCR to quantify mitochondrial DNA (mtDNA) copy numbers in three different tissues types: breast, colon and prostate. For each type, normal tissue and a tumor from the same three patients were analysed. This gives a total of six samples. The mitochondrial copy number is estimated to lie between 200 and 300 copies per cell. Similar results are obtained when using the robust regression or the [Formula: see text] method. Confidence ratios were slightly narrower for the robust regression. The new data analysis method has been implemented as an R package.
Keyphrases
- mitochondrial dna
- copy number
- genome wide
- data analysis
- real time pcr
- end stage renal disease
- dna methylation
- prostate cancer
- chronic kidney disease
- ejection fraction
- human milk
- oxidative stress
- newly diagnosed
- smoking cessation
- stem cells
- peritoneal dialysis
- single cell
- gene expression
- liquid chromatography tandem mass spectrometry
- low birth weight
- benign prostatic hyperplasia