Gold Nanoparticle Smartphone Platform for Diagnosing Urinary Tract Infections.
Kyryl ZagorovskyMaria Teresa Fernández-ArgüellesDiane BonaAshraf Mohamed ElshawadfyAbdullah Muhammad SyedPranav KadhiresanTony MazzulliKaren L MaxwellWarren W C ChanPublished in: ACS nanoscience Au (2022)
Current urinary tract infection (UTI) diagnostic methods are slow or provide limited information, resulting in prescribing antibiotic therapy before bacterial pathogen identification. Here, we adapted a gold nanoparticle colorimetric approach and developed a smartphone platform for UTI detection. We show the parallel identification of five major UTI pathogens at clinically relevant concentrations of 10 5 bacteria/mL using bacteria-specific and universal probes. We validated the diagnostic technology using 115 positive and 19 negative samples from patients with Escherichia coli , Proteus mirabilis, and Klebsiella pneumoniae infections. The assay successfully identified the infecting pathogen (specificity: >98% and sensitivity: 51-73%) in 3 h. Our platform is faster than culturing and can wirelessly store and transmit results at the cost of $0.38 per assay.
Keyphrases
- urinary tract infection
- klebsiella pneumoniae
- high throughput
- escherichia coli
- multidrug resistant
- primary care
- candida albicans
- gold nanoparticles
- living cells
- small molecule
- gram negative
- bioinformatics analysis
- single cell
- label free
- sensitive detection
- biofilm formation
- nitric oxide
- loop mediated isothermal amplification
- cystic fibrosis
- staphylococcus aureus
- social media
- structural basis