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Gold Nanoparticle Smartphone Platform for Diagnosing Urinary Tract Infections.

Kyryl ZagorovskyMaria Teresa Fernández-ArgüellesDiane BonaAshraf Mohamed ElshawadfyAbdullah Muhammad SyedPranav KadhiresanTony MazzulliKaren L MaxwellWarren W C Chan
Published in: ACS nanoscience Au (2022)
Current urinary tract infection (UTI) diagnostic methods are slow or provide limited information, resulting in prescribing antibiotic therapy before bacterial pathogen identification. Here, we adapted a gold nanoparticle colorimetric approach and developed a smartphone platform for UTI detection. We show the parallel identification of five major UTI pathogens at clinically relevant concentrations of 10 5 bacteria/mL using bacteria-specific and universal probes. We validated the diagnostic technology using 115 positive and 19 negative samples from patients with Escherichia coli , Proteus mirabilis, and Klebsiella pneumoniae infections. The assay successfully identified the infecting pathogen (specificity: >98% and sensitivity: 51-73%) in 3 h. Our platform is faster than culturing and can wirelessly store and transmit results at the cost of $0.38 per assay.
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