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Synthetic Dimerization Approaches for In Vivo Studies in Saccharomyces cerevisiae.

Jana ElsaesserClaudine Kraft
Published in: Methods in molecular biology (Clifton, N.J.) (2024)
Synthetic tethering approaches induced by chemical means offer precise control over protein interactions in cells. They enable the manipulation of when, where, and how proteins interact, making it possible to study their functions, dynamics, and cellular consequences at a molecular level. These methods are versatile, reversible, and adaptable, allowing the dissection of complex cellular processes and the engineering of cellular functions. Here, we describe two chemically induced dimerization systems in the model organism Saccharomyces cerevisiae. Using the autophagy pathway as an example, we show how these approaches can be used to dissect molecular events in cells.
Keyphrases
  • saccharomyces cerevisiae
  • induced apoptosis
  • cell cycle arrest
  • cell death
  • signaling pathway
  • oxidative stress
  • diabetic rats
  • small molecule
  • protein protein
  • amino acid
  • stress induced