Cytotoxicity of Hybrid Noble Metal-Polymer Composites.
Anton S TkachenkoPavlo A VirychValeriy MyasoyedovVolodymyr ProkopiukAnatolii OnishchenkoDmytro ButovYuliia I KuzivOleg A YeshchenkoSican ZhongGuochao NieNataliya V KutsevolPublished in: BioMed research international (2022)
The aim of the present research was to assess the cytotoxicity of gold and silver nanoparticles synthesized into dextran-graft-polyacrylamide (D-PAA) polymer nanocarrier, which were used as a basis for further preparation of multicomponent nanocomposites revealed high efficacy for antitumor therapy. The evaluation of the influence of Me-polymer systems on the viability and metabolic activity of fibroblasts and eryptosis elucidating the mechanisms of the proeryptotic effects has been done in the current research. The nanocomposites investigated in this study did not reduce the survival of fibroblasts even at the highest used concentration. Our findings suggest that hybrid Ag/D-PAA composite activated eryptosis via ROS- and Ca 2+ -mediated pathways at the low concentration, in contrast to other studied materials. Thus, the cytotoxicity of Ag/D-PAA composite against erythrocytes was more pronounced compared with D-PAA and hybrid Au/polymer composite. Eryptosis is a more sensitive tool for assessing the biocompatibility of nanomaterials compared with fibroblast viability assays.
Keyphrases
- silver nanoparticles
- visible light
- reduced graphene oxide
- quantum dots
- magnetic resonance
- drug delivery
- cell death
- extracellular matrix
- highly efficient
- sensitive detection
- gold nanoparticles
- carbon nanotubes
- oxidative stress
- cell therapy
- mesenchymal stem cells
- protein kinase
- molecularly imprinted
- free survival
- contrast enhanced
- solid phase extraction