Metabolic engineering of Escherichia coli to improve recombinant protein production.
Min LiuXinjun FengYamei DingGuang ZhaoHuizhou LiuGuang ZhaoPublished in: Applied microbiology and biotechnology (2015)
Escherichia coli is one of the most widely used strains for recombinant protein production. However, obstacles also exist in both academic researches and industrial applications, such as the metabolic burden, the carbon source waste, and the cells' physiological deterioration. This article reviews recent approaches for improving recombinant protein production in metabolic engineering, including workhorse selection, stress factor application, and carbon flux regulation. Selecting a suitable host is the first key point for recombinant protein production. In general, it all depends on characteristics of the strains and the target proteins. It will be triggered cells physiological deterioration when the medium is significantly different from the cell's natural environment. Coexpression of stress factors can help proteins to fold into their native conformation. Carbon flux regulation is a direct approach for redirecting more carbon flux toward the desirable pathways and products. However, some undesirable consequences are usually found in metabolic engineering, such as glucose transport inhibition, cell growth retardation, and useless metabolite accumulation. More efficient regulators and platform cell factories should be explored to meet a variety of production demands.
Keyphrases
- escherichia coli
- induced apoptosis
- protein protein
- single cell
- heavy metals
- binding protein
- amino acid
- cell cycle arrest
- cell free
- stem cells
- transcription factor
- cell therapy
- type diabetes
- blood pressure
- klebsiella pneumoniae
- risk factors
- bone marrow
- skeletal muscle
- staphylococcus aureus
- small molecule
- biofilm formation
- glycemic control
- mesenchymal stem cells