Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging.

Stephen D Grant Gemma S CairnsJordan WistubaBrian R Patton

Published in: F1000Research (2019)

We report on a 3D printed microscope, based on a design by the Openflexure project, that uses low cost components to perform fluorescence imaging. The system is sufficiently sensitive and mechanically stable to allow the use of the Super Resolution Radial Fluctuations algorithm to obtain images with resolution better than the diffraction limit. Due to the low-cost components, the entire system can be built for approximately $1200.

Keyphrases

low cost
fluorescence imaging
deep learning
photodynamic therapy
machine learning
quality improvement
high resolution
convolutional neural network
single molecule
ultrasound guided
crystal structure
electron microscopy