Expression of TNF, IL1B, and iNOS2 in the neural cell after induced by Porphyromonas gingivalis with and without coating antibody anti -Porphyromonas gingivalis.
Endang Winiati BachtiarCitra F PutriRetno D SoejoedonoBoy Muchlis BachtiarPublished in: F1000Research (2020)
Porphyromonas gingivalis has virulence factors such as gingipain and lipopolysaccharide, causing bacteremia to reach the brain and activate neuroinflammatory release cytokines. This study analyzed the effect of the co-culture of neuron cells with P. gingivalis coated with anti -P. gingivalis antibodies against cytokines produced by neuron cells. The gene expressions of the TNF, IL1B, iNOS2 in neurons was evaluated using RT-qPCR. The results showed that P. gingivalis coated with anti -P. gingivalis antibody before co-culture with neuron cells could decrease the gene expression of TNF, IL1B, and iNOS2 of neuron cells.
Keyphrases
- induced apoptosis
- gene expression
- cell cycle arrest
- rheumatoid arthritis
- nitric oxide
- multiple sclerosis
- endoplasmic reticulum stress
- stem cells
- single cell
- signaling pathway
- spinal cord
- spinal cord injury
- long non coding rna
- transcription factor
- mesenchymal stem cells
- cell proliferation
- nitric oxide synthase
- antimicrobial resistance
- resting state