Green micellar spectrofluorimetric determination of α-mangostin as antioxidant and other biological activities beneficial to human health found in herbal products.

Pharmaceutical product quality control (QC) needs for quick, sensitive, and economical procedures in order to deliver high throughput at low cost, which is the key factor taken into account by such economic facilities. To lessen the risky effects of research laboratories, researchers must take into account the ecological impacts. Anti-inflammatory, antioxidant, anticancer, anti-allergic, antibacterial, antifungal, antiviral, and antimalarial activities are all possessed by α-mangostin (MAG). Based on spectrofluorimetric approach, a novel straightforward, sensitive, and environmentally friendly method for MAG determination was developed and validated. Numerous variables were investigated to enhance MAG native fluorescence, including solvent type, buffering, pH, and additional surfactants. The best MAG fluorescence sensitivity was found in Britton-Robinson buffer (pH 4) at 450 nm following irradiation at 350 nm in the concentration range of 5-50 ng mL-1. The technique was successfully used to determine the presence of MAG in both its approved dose forms and in samples of spiked human plasma, as per FDA standards for validation. According to their evaluation on two recent greenness criteria (GAPI and AGREE), the suggested approach has been shown to be environmentally beneficial because it normally uses bio degradable chemicals in solvent-free aqueous phases.