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Cationic guanine: positively charged nucleobase with improved DNA affinity inhibits self-duplex formation.

Masaki HibinoYuichiro AibaOsami Shoji
Published in: Chemical communications (Cambridge, England) (2020)
Oligonucleotides represent powerful DNA-recognition tools, but the formation of undesirable "self-duplexes" becomes more probable with increasing DNA affinity. Herein, we have developed a modified nucleobase with "self-avoiding" properties. Facile methylation of guanine yields a cationic N7-methylguanine, which suppresses the formation of self-duplexes whilst improving DNA affinity through electrostatic interaction.
Keyphrases
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  • mass spectrometry