Investigation of polymorphisms in BMP2, BMP4, SMAD6 and RUNX2 genes and pulp stones.
Katherine Azevedo Batistela Rodrigues ThullerLivia Azeredo Alves AntunesPrescila Mota de Oliveira KublitskiJoão Armando BrancherFlares Baratto FilhoErika Calvano KüchlerAlice Corrêa Silva-SousaManoel Damião de Sousa-NetoMarilisa Carneiro Leão GabardoLeonardo Dos Santos AntunesPublished in: Australian endodontic journal : the journal of the Australian Society of Endodontology Inc (2024)
This study aimed to assess the association between genetic polymorphisms in BMP2 (rs1005464 and rs235768), BMP4 (rs17563), SMAD6 (rs2119261 and rs3934908) and RUNX2 (rs59983488 and rs1200425) and pulp stones (PS). A total of 117 participants, consisting of 63 individuals with PS and 54 without PS, were included. Digital radiographs and a demographic/clinical questionnaire were used. Genomic DNA from salivary cells was genotyped via real-time polymerase chain reaction. Statistical analyses, including Chi-Square, Fisher's exact tests, Poisson regression and dimensionality reduction, were conducted. The rs2119261 polymorphism in the SMAD6 gene showed an association with genotype distribution in the recessive model (p = 0.049). The T-T haplotype in the SMAD6 gene (rs2119261 and rs3934908) was more prevalent in the control group and significantly linked with PS (p = 0.029). No associations were found between PS risk and genetic polymorphisms in BMP2, BMP4 and RUNX2. Polymorphisms in the SMAD6 gene were associated with PS.
Keyphrases
- mesenchymal stem cells
- epithelial mesenchymal transition
- transforming growth factor
- genome wide
- copy number
- transcription factor
- bone regeneration
- genome wide identification
- induced apoptosis
- gene expression
- bone marrow
- cell proliferation
- autism spectrum disorder
- duchenne muscular dystrophy
- molecular dynamics
- signaling pathway
- patient reported