Rapid reconstitution of ubiquitinated nucleosome using a non-denatured histone octamer ubiquitylation approach.
Weijie LiPeirong CaoPengqi XuFahui SunChi WangJiale ZhangShuqi DongJon R WilsonDifei XuHengxin FanZhenhuan FengXiaofei ZhangQingjun ZhuYingzhi FanNick BrownNeil JustinSteven J GamblinHe LiYing ZhangJun HePublished in: Cell & bioscience (2024)
Overall, our findings offer an efficient strategy for producing ubiquitinated nucleosomes, advancing biochemical and biophysical studies in the field of chromatin biology.