Highly Effective and Noninvasive Near-Infrared Eradication of a Staphylococcus aureus Biofilm on Implants by a Photoresponsive Coating within 20 Min.
Mu LiLiqian LiKun SuXiangmei LiuTianjin ZhangYanqin LiangDoudou JingXianjin YangDong ZhengZhenduo CuiZhaoyang LiShengli ZhuKelvin Wai Kwok YeungYufeng ZhengXianbao WangShui-Lin WuPublished in: Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2019)
Biofilms have been related to the persistence of infections on medical implants, and these cannot be eradicated because of the resistance of biofilm structures. Therefore, a biocompatible phototherapeutic system is developed composed of MoS2, IR780 photosensitizer, and arginine-glycine-aspartic acid-cysteine (RGDC) to safely eradicate biofilms on titanium implants within 20 min. The magnetron-sputtered MoS2 film possesses excellent photothermal properties, and IR780 can produce reactive oxygen species (ROS) with the irradiation of near-infrared (NIR, λ = 700-1100 nm) light. Consequently, the combination of photothermal therapy (PTT) and photodynamic therapy (PDT), assisted by glutathione oxidation accelerated by NIR light, can provide synergistic and rapid killing of bacteria, i.e., 98.99 ± 0.42% eradication ratio against a Staphylococcus aureus biofilm in vivo within 20 min, which is much greater than that of PTT or PDT alone. With the assistance of ROS, the permeability of damaged bacterial membranes increases, and the damaged bacterial membranes become more sensitive to heat, thus accelerating the leakage of proteins from the bacteria. In addition, RGDC can provide excellent biosafety and osteoconductivity, which is confirmed by in vivo animal experiments.
Keyphrases
- photodynamic therapy
- staphylococcus aureus
- reactive oxygen species
- candida albicans
- biofilm formation
- fluorescence imaging
- room temperature
- soft tissue
- reduced graphene oxide
- quantum dots
- helicobacter pylori infection
- cell death
- methicillin resistant staphylococcus aureus
- pseudomonas aeruginosa
- dna damage
- healthcare
- nitric oxide
- visible light
- ionic liquid
- high resolution
- heat stress
- transition metal
- oxidative stress
- escherichia coli
- radiation induced
- amino acid
- helicobacter pylori
- single molecule
- drug induced
- loop mediated isothermal amplification