Analysis of Chirality Effects on Stem Cell Fate Using Three-dimensional Fibrous Peptide Hydrogels.
Hangyu ZhengToru YoshitomiKeitaro YoshimotoPublished in: ACS applied bio materials (2018)
Chirality effects on stem cell fate were investigated in three-dimensional culture using soft fibrous hydrogels consisting of self-assembled l - and d -form Fmoc-Phe-Phe-Cys networks photo-cross-linked by poly(ethylene glycol) ( l - and d -gel, respectively). Encapsulated human bone marrow-derived mesenchymal stem cells were all alive, spread, and grown in both hydrogels. Interestingly, the cells preferably spread and grew in l -gel compared to in d -gel under mixed induction, and cell osteogenesis and adipogenesis differentiation in d -gel were suppressed compared to in l -gel. These results revealed that stem cell function and fate can be regulated in three-dimensional hydrogel culture systems with chiral motifs.
Keyphrases
- hyaluronic acid
- wound healing
- stem cells
- drug delivery
- bone marrow
- single cell
- tissue engineering
- endothelial cells
- extracellular matrix
- cell therapy
- drug release
- cell cycle arrest
- metabolic syndrome
- adipose tissue
- insulin resistance
- cell proliferation
- skeletal muscle
- endoplasmic reticulum stress
- atomic force microscopy