Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation.
Dominique C StephensMargaret MungaiAmber CrabtreeHeather K BeasleyEdgar Garza-LopezLarry VangKit NeikirkZer VueNeng VueAndrea G MarshallKyrin TurnerJian-Qiang ShaoBishnu SarkerSandra MurrayJennifer A GaddyJamaine DavisSteven M DamoAntentor O HintonPublished in: bioRxiv : the preprint server for biology (2023)
Myoblasts are isolated from mouse limb muscles. They are plated for immunofluorescence-based validation using confocal microscopy. This method demonstrates the need for reliable antibodies to correctly determine and differentiate between cellular populations within isolated skeletal muscles.