DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection.
Lin QiMingshu XiaoXiwei WangCheng WangLihua WangShiping SongXiangmeng QuLi LiJiye ShiHao PeiPublished in: Analytical chemistry (2017)
The development of highly sensitive and selective methods for the detection of microRNA (miRNA) has attracted tremendous attention because of its importance in fundamental biological studies and diagnostic applications. In this work, we develop DNA-encoded Raman-active anisotropic nanoparticles modified origami paper analytical devices (oPADs) for rapid, highly sensitive, and specific miRNA detection. The Raman-active anisotropic nanoparticles were prepared using 10-mer oligo-A, -T, -C, and -G to mediate the growth of Ag cubic seeds into Ag nanoparticles (AgNPs) with different morphologies. The resulting AgNPs were further encoded with DNA probes to serve as effective surface-enhanced Raman scattering (SERS) probes. The analytical device was then fabricated on a single piece of SERS probes loaded paper-based substrate and assembled based on the principles of origami. The addition of the target analyte amplifies the Raman signals on DNA-encoded AgNPs through a target-dependent, sequence specific DNA hybridization assembly. This simple and low-cost analytical device is generic and applicable to a variety of miRNAs, allowing detection sensitivity down to 1 pM and assay time within 15 min, and therefore holds promising applications in point-of-care diagnostics.
Keyphrases
- label free
- single molecule
- circulating tumor
- loop mediated isothermal amplification
- nucleic acid
- cell free
- living cells
- raman spectroscopy
- small molecule
- low cost
- sensitive detection
- gold nanoparticles
- real time pcr
- fluorescence imaging
- drug delivery
- circulating tumor cells
- silver nanoparticles
- quantum dots
- particulate matter
- working memory
- highly efficient
- high resolution
- mass spectrometry
- finite element
- risk assessment
- molecularly imprinted
- case control