UHRF1 depletion and HDAC inhibition reactivate epigenetically silenced genes in colorectal cancer cells.

Takeshi NiinumaHiroshi KitajimaMasahiro KaiEiichiro YamamotoAkira YorozuKazuya IshiguroHajime SasakiGota SudoMutsumi ToyotaTomo HatahiraReo MaruyamaTakashi TokinoHiroshi NakaseTamotsu SugaiHiromu Suzuki

Published in: Clinical epigenetics (2019)

Our results suggest that (i) maintenance of DNA methylation in CRC cells is highly dependent on UHRF1; (ii) UHRF1 depletion rapidly induces DNA demethylation, though it is insufficient to fully reactivate the silenced genes; and (iii) dual targeting of UHRF1 and HDAC may be an effective new therapeutic strategy.

Keyphrases

genome wide
dna methylation
induced apoptosis
histone deacetylase
gene expression
bioinformatics analysis
cell cycle arrest
genome wide identification
circulating tumor
transcription factor
oxidative stress
long noncoding rna
drug delivery
cell proliferation