Modified Graphite Pencil Electrode Based on Graphene Oxide-Modified Fe 3 O 4 for Ferrocene-Mediated Electrochemical Detection of Hemoglobin.

This study describes the synthesis of graphene oxide-modified magnetite (rGO/Fe 3 O 4 ) and its use as an electrochemical sensor for the quantitative detection of hemoglobin (Hb). rGO is characterized by a 2θ peak at 10.03° in its X-ray diffraction, 1353 and 1586 cm -1 vibrations in Raman spectroscopy, while scanning electron microscopy coupled with energy-dispersive spectroscopy of rGO and rGO/Fe 3 O 4 revealed the presence of microplate structures in both materials and high presence of iron in rGO/Fe 3 O 4 with 50 wt %. The modified graphite pencil electrode, GPE/rGO/Fe 3 O 4 , is characterized using cyclic voltammetry. Higher electrochemical surface area is obtained when the GPE is modified with rGO/Fe 3 O 4 . Linear scan voltammetry is used to quantify Hb at the surface of the sensor using ferrocene (FC) as an electrochemical amplifier. Linear response for Hb is obtained in the 0.1-1.8 μM range with a regression coefficient of 0.995, a lower limit of detection of 0.090 μM, and a limit of quantitation of 0.28 μM. The sensor was free from interferents and successfully used to sense Hb in human urine. Due to the above-stated qualities, the GPE/rGO/Fe 3 O 4 electrode could be a potential competitive sensor for trace quantities of Hb in physiological media.