Multispectral optoacoustic tomography is more sensitive than micro-computed tomography for tracking gold nanorod labelled mesenchymal stromal cells.
Alejandra Hernandez PichardoJames LittlewoodArthur TaylorBettina WilmRaphaël LévyPatricia MurrayPublished in: Journal of biophotonics (2023)
Tracking the fate of therapeutic cell types is important for assessing their safety and efficacy. Bioluminescence imaging (BLI) is an effective cell tracking technique, but poor spatial resolution means it has limited ability to precisely map cells in vivo in 3D. This can be overcome by using a bimodal imaging approach that combines BLI with a technique capable of generating high resolution images. Here we compared the effectiveness of combining either multispectral optoacoustic tomography (MSOT) or micro-computed tomography (micro-CT) with BLI for tracking the fate of luciferase + human mesenchymal stromal cells (MSCs) labelled with gold nanorods. Following subcutaneous administration in mice, the MSCs could be readily detected with MSOT but not with micro-CT. We conclude that MSOT is more sensitive than micro-CT for tracking gold nanorod-labelled cells in vivo and depending on the route of administration, can be used effectively with BLI to track MSC fate in mice.
Keyphrases
- computed tomography
- dual energy
- high resolution
- image quality
- positron emission tomography
- contrast enhanced
- induced apoptosis
- magnetic resonance imaging
- mesenchymal stem cells
- single cell
- bone marrow
- cell cycle arrest
- endothelial cells
- cell therapy
- high fat diet induced
- systematic review
- magnetic resonance
- silver nanoparticles
- mass spectrometry
- umbilical cord
- oxidative stress
- deep learning
- type diabetes
- machine learning
- convolutional neural network
- insulin resistance
- optical coherence tomography
- reduced graphene oxide
- high speed