Login / Signup

Use of FTA card for the detection of two RNA (CSFV and SV-A) and two DNA viruses (ASFVand SuHV-1) of importance in veterinary medicine.

Antônio Augusto Fonseca JúniorValdênia Lopes da Silva GonçalvesAline Aparecida Silva BarbosaMarcelo Fernandes Camargos
Published in: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2024)
The FTA card has emerged as a promising alternative for nucleic acid extraction. The FTA card is a filter paper impregnated with chemicals that preserve and stabilize the genetic material present in the sample, allowing for its storage and transport at room temperature. The aim of this study was to test the card for the detection of RNA and DNA nucleic acids. Two RNA viruses (Senecavirus A and classical swine fever virus) and two DNA viruses (African swine fever virus and suid alphaherpesvirus 1) were tested, and in all cases, there was a decrease in sensitivity. The methods exhibited good repeatability and demonstrated a rapid and practical use for sample transport and nucleic acid extraction.
Keyphrases
  • nucleic acid
  • room temperature
  • loop mediated isothermal amplification
  • label free
  • real time pcr
  • circulating tumor
  • ionic liquid
  • genome wide
  • gene expression
  • dna methylation
  • copy number
  • cell free
  • single molecule