Stability Assessment of Candidate Reference Genes in Urine Sediment of Prostate Cancer Patients for miRNA Applications.
Maria Giulia EgidiGiovanni CochettiGabriella GuelfiDanilo ZampiniSilvana DiverioGiulia PoliEttore MeariniPublished in: Disease markers (2015)
We aimed at assessing the stability of candidate reference genes in urine sediments of men subjected to digital rectal examination for suspected prostate cancer (PCa). Two microRNAs (miR-191 and miR-25) and 1 small nucleolar RNA (SNORD48) were assayed in 35 post-DRE urine sediments of men with PCa and in 26 subjects with histologically confirmed benign prostatic hyperplasia (BPH). The stability of candidate reference genes was assessed through BestKeeper algorithm and equivalence test. miR-200b and miR-452 were used to test for the effect of normalization on target genes. Our results proved miR-191 to be the most stable gene, showing the lowest degree of variation and the highest stability value. miR-25 and SNORD48 values fell beyond the cutoff of acceptability. In conclusion, we recommend the use of miR-191 for normalization purposes in post-DRE urine sediments.
Keyphrases
- cell proliferation
- long non coding rna
- prostate cancer
- long noncoding rna
- heavy metals
- genome wide
- benign prostatic hyperplasia
- genome wide identification
- lower urinary tract symptoms
- end stage renal disease
- newly diagnosed
- radical prostatectomy
- bioinformatics analysis
- chronic kidney disease
- polycyclic aromatic hydrocarbons
- gene expression
- ejection fraction
- dna methylation
- copy number