Somatic embryogenesis and β-glucuronidase transformation in chickpea (Cicer arietinum cv. Bivanich).
Mohammad Reza AmaniAlireza ZebarjadiDanial KahriziSezai ErcisliPublished in: Molecular biology reports (2022)
The best environment for regeneration embryos was MS medium with 2.5 mg/l of 2,4-D concentration gradient to zero. In this study, the PCR reaction showed the presence of the β-glucuronidase (gus) marker gene in regenerated cotyledons for 20 min in all three strains studied.