Highly Sensitive Detection of PQS Quorum Sensing in Pseudomonas Aeruginosa Using Screen-Printed Electrodes Modified with Nanomaterials.
Denisa CapatinaTeodora LupoiBogdan FeierDiana OlahCecilia CristeaRadu OpreanPublished in: Biosensors (2022)
The rapid diagnosis of Pseudomonas aeruginosa infection is very important because this bacterium is one of the main sources of healthcare-associated infections. Pseudomonas quinolone signal (PQS) is a specific molecule for quorum sensing (QS) in P. aeruginosa , a form of cell-to-cell bacterial communication and its levels can allow the determination of the bacterial population. In this study, the development of the first electrochemical detection of PQS using screen-printed electrodes modified with carbon nanotubes (CNT-SPE) is reported. The electrochemical fingerprint of PQS was determined using different electrode materials and screen-printed electrodes modified with different nanomaterials. The optimization of the method in terms of electrolyte, pH, and electrochemical technique was achieved. The quantification of PQS was performed using one of the anodic peaks in the electrochemical fingerprint of the PQS on the CNT-SPE. The sensor exhibited a linear range from 0.1 to 15 µM, with a limit of detection of 50 nM. The sensor allowed the selective detection of PQS, with low interference from other QS molecules. The sensor was successfully applied to analysis of real samples (spiked urine and human serum samples, spiked microbiological growth media, and microbiological cultures).
Keyphrases
- label free
- carbon nanotubes
- loop mediated isothermal amplification
- pseudomonas aeruginosa
- molecularly imprinted
- gold nanoparticles
- ionic liquid
- healthcare
- solid phase extraction
- real time pcr
- cystic fibrosis
- single cell
- solid state
- biofilm formation
- cell therapy
- photodynamic therapy
- acinetobacter baumannii
- escherichia coli
- sensitive detection
- stem cells
- social media
- staphylococcus aureus
- multidrug resistant
- electron transfer