Diagnosis of Alzheimer's Disease and In Situ Biological Imaging via an Activatable Near-Infrared Fluorescence Probe.
Yuexia YangLiang ZhangJiemin WangYuping CaoShuyan LiWenwu QinYun LiuPublished in: Analytical chemistry (2022)
Alzheimer's disease (AD) is a common neurodegenerative disease that makes the brain nervous system degenerate rapidly and is accompanied by some special cognitive and behavioral dysfunction. Recently, butyrylcholinesterase ( BChE ) was reported as an important enzyme, whose activity can provide predictive value for timely discovery and diagnosis of AD. Therefore, it is indispensable to design a detection tool for selective and rapid response toward BChE . In this study, we developed a novel near-infrared fluorescent probe ( Chy-1 ) for the detection of BChE activity. An excellent sensitivity, good biocompatibility, and lower limit of detection (LOD) of 0.12 ng/mL made the probe extremely specific for BChE , which was successfully used in biological imaging. What is more, Chy-1 can not only clearly distinguish tumor from normal cells but also forms a clear boundary between the normal and cancer tissues due to the obvious difference in fluorescence intensity produced via in situ spraying. Most important of all, Chy-1 was also successfully applied to track the BChE activity in AD mouse models. Based on this research, the novel probe may be a powerful tool for clinical diagnosis and therapy of tumor and neurodegenerative diseases.
Keyphrases
- living cells
- fluorescent probe
- loop mediated isothermal amplification
- high resolution
- single molecule
- quantum dots
- mouse model
- cognitive decline
- induced apoptosis
- stem cells
- squamous cell carcinoma
- oxidative stress
- high throughput
- papillary thyroid
- mass spectrometry
- sensitive detection
- fluorescence imaging
- white matter
- signaling pathway
- single cell
- energy transfer
- endoplasmic reticulum stress
- subarachnoid hemorrhage
- blood brain barrier
- resting state