DNA methylation analysis identifies key transcription factors involved in mesenchymal stem cell osteogenic differentiation.
Rodolfo GomezMatt J BarterAna Alonso-PérezAndrew J SkeltonCarole ProctorGabriel Herrero-BeaumontDavid A YoungPublished in: Biological research (2023)
In this work we describe an osteoblastogenesis-associated DNA-methylation profile and, using these data, validate a novel computational tool to identify key TFs associated to age-related disease processes. By means of this tool we identified and confirmed ZEB TFs as mediators involved in the MSCs differentiation to osteoblasts and adipocytes, and obesity-related bone adiposity.
Keyphrases
- dna methylation
- mesenchymal stem cells
- genome wide
- insulin resistance
- umbilical cord
- bone marrow
- transcription factor
- gene expression
- high fat diet induced
- weight gain
- adipose tissue
- metabolic syndrome
- type diabetes
- weight loss
- epithelial mesenchymal transition
- copy number
- cell therapy
- long non coding rna
- electronic health record
- big data
- stem cells
- body mass index
- soft tissue
- postmenopausal women
- bone regeneration