Kilohertz two-photon brain imaging in awake mice.
Tong ZhangOscar HernandezRadosław ChrapkiewiczAdam ShaiMark J WagnerYanping ZhangCheng-Hsun WuJin Zhong LiMasatoshi InoueYiyang GongBiafra AhanonuHongkui ZengHaruhiko BitoMark J SchnitzerPublished in: Nature methods (2019)
Two-photon microscopy is a mainstay technique for imaging in scattering media and normally provides frame-acquisition rates of ~10-30 Hz. To track high-speed phenomena, we created a two-photon microscope with 400 illumination beams that collectively sample 95,000-211,000 µm2 areas at rates up to 1 kHz. Using this microscope, we visualized microcirculatory flow, fast venous constrictions and neuronal Ca2+ spiking with millisecond-scale timing resolution in the brains of awake mice.
Keyphrases
- high speed
- high resolution
- atomic force microscopy
- living cells
- high fat diet induced
- monte carlo
- deep brain stimulation
- cerebral ischemia
- high frequency
- resting state
- mass spectrometry
- white matter
- wild type
- high throughput
- functional connectivity
- adipose tissue
- protein kinase
- single cell
- optical coherence tomography