Oxidative stress induces release of mitochondrial DNA into the extracellular space in human placental villous trophoblast BeWo cells.
Jennifer J GardnerSpencer C CushenRenée de Nazaré Oliveira da SilvaJessica L BradshawNataliia HulaIsabelle K GorhamSelina M TuckerZhengyang ZhouRebecca L CunninghamNicole R PhillipsStyliani GoulopoulouPublished in: bioRxiv : the preprint server for biology (2024)
This is the first study to test whether trophoblast cells release mitochondrial DNA in response to oxidative stress and to identify mechanisms of release and biological forms of mtDNA from this cellular type. This research identifies potential cellular mechanisms that can be used in future investigations to establish the source and biomarker potential of circulating mitochondrial DNA in preclinical experimental models and humans.
Keyphrases
- mitochondrial dna
- copy number
- induced apoptosis
- oxidative stress
- cell cycle arrest
- endoplasmic reticulum stress
- genome wide
- signaling pathway
- endothelial cells
- dna damage
- ischemia reperfusion injury
- gene expression
- cell death
- stem cells
- mesenchymal stem cells
- cell proliferation
- risk assessment
- induced pluripotent stem cells
- heat shock