Use of Cyclodextrin as a Novel Agent in the SEC-HPLC Mobile Phase to Mitigate the Interactions of Proteins or Peptide or their Impurities with the Residual Silanols of Commercial SEC-HPLC Columns with Improved Separation and Resolution.
Indu JaveriKaliappanadar NellaiappanCharles McNemarKirill YakovlevskyAmina SoukratiPhanindra VelisettyBijay MisraPublished in: Pharmaceutical research (2018)
Hydroxypropyl ß Cyclodextrin in the mobile phase, resulted improved SEC-HPLC resolution, and quantitation of aggregates in biologics by preventing the interaction of biologics to silanol of the commercial SEC-HPLC columns.