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Biological Nanopores: Engineering on Demand.

Ana CrnkovićMarija SrnkoGregor Anderluh
Published in: Life (Basel, Switzerland) (2021)
Nanopore-based sensing is a powerful technique for the detection of diverse organic and inorganic molecules, long-read sequencing of nucleic acids, and single-molecule analyses of enzymatic reactions. Selected from natural sources, protein-based nanopores enable rapid, label-free detection of analytes. Furthermore, these proteins are easy to produce, form pores with defined sizes, and can be easily manipulated with standard molecular biology techniques. The range of possible analytes can be extended by using externally added adapter molecules. Here, we provide an overview of current nanopore applications with a focus on engineering strategies and solutions.
Keyphrases
  • single molecule
  • label free
  • loop mediated isothermal amplification
  • living cells
  • atomic force microscopy
  • drinking water
  • hydrogen peroxide
  • single cell
  • real time pcr
  • water soluble
  • small molecule
  • sensitive detection