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Nanopore PCR-cDNA sequencing of the biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) informs gene annotation.

Andrew Wesley LeganHillary L MehlAlex VaraksaKenneth A Callicott
Published in: Microbiology resource announcements (2023)
Toxic molds in the Aspergillus genus synthesize carcinogenic aflatoxins which contaminate crops. The widely applied biocontrol isolate Aspergillus flavus AF36 (NRRL 18543) has a high-quality public genome but lacks corresponding gene annotations. We generated high-quality gene predictions for this isolate by using long-read Nanopore PCR-cDNA sequencing.
Keyphrases
  • genome wide
  • copy number
  • single molecule
  • atrial fibrillation
  • single cell
  • genome wide identification
  • healthcare
  • cell wall
  • mental health
  • emergency department
  • rna seq
  • real time pcr