Virulence Genes Profile and Antimicrobial Susceptibility of Community-Acquired Bacterial Urinary Tract Infections in a Brazilian Hospital.
Fernanda Morcatti CouraVitoria Marra de Souza SaviniRafael Gariglio Clark XavierCarolina Pantuzza RamosRodrigo Otavio Silveira SilvaMarcos Bryan HeinemannAndrea Micke MorenoTerezinha KnöblElaine Maria Seles DornelesDirceia Aparecida da Costa CustódioCarine Rodrigues PereiraPedro Felipe Rodrigues E OliveiraAmanda Bruno PulinelliPublished in: Current microbiology (2021)
Urinary tract infections (UTI) are one of the most common diseases worldwide and Escherichia coli is the most common causative bacteria. Empirical treatment is challenging due to antimicrobial or multidrug-resistance. The aims of this study were to determine the uropathogens and their antimicrobial susceptibility profile, as well as to identify the phylogroups and virulence genes of E. coli strains, associated with community-acquired UTI in outpatients admitted at a Brazilian Hospital in southeast Brazil. In total, 47 bacterial strains were isolated from 47 patients, 44 women and 2 men (no gender record from one patient). The age of the patients whose urine culture were positive varied from 0 (less than one month) to 104 years. Most of the isolates were E. coli (41/47), followed by Klebsiella pneumoniae (2/47), Klebsiella variicola/Klebsiella aerogenes (1/47), Pseudomonas aeruginosa (1/47), Proteus mirabilis (1/47), and Citrobacter koseri (1/47). Most E. coli strains were classified as phylogroup B2 (15/41 = 36.59%) and B1 (12/41 = 29.27%) and the most common virulence genes among E. coli strains were fimH (31/41 = 75.61%), iutA (21/41 = 51.22%), and tratT (16/41 = 39.02%). Among the E. coli strains, 59% were multidrug-resistance and strains that were ampicillin, sulfamethoxazole/trimethoprim, or tetracycline-resistant exhibited more chance to be multidrug-resistance, with an odds ratio of 100.00 [95% confidence interval (CI) 9.44-1059.26], 22.50 (95% CI 3.95-128.30), and 12.83 (95% CI 2.68-61.45), respectively. Our results showed that E. coli was the main etiological agent identified and demonstrated high frequency of multidrug-resistance and virulence factors in bacterial strains isolated from UTIs.
Keyphrases
- escherichia coli
- urinary tract infection
- klebsiella pneumoniae
- biofilm formation
- pseudomonas aeruginosa
- high frequency
- end stage renal disease
- ejection fraction
- healthcare
- newly diagnosed
- chronic kidney disease
- staphylococcus aureus
- genome wide
- peritoneal dialysis
- cystic fibrosis
- prognostic factors
- pregnant women
- patient reported outcomes
- adipose tissue
- multidrug resistant
- bioinformatics analysis
- adverse drug
- combination therapy
- acute care
- genome wide analysis
- drug induced
- insulin resistance