Persistent luminescence (PL) bioimaging provides an optimal method of eliminating autofluorescence for a higher resolution and sensitivity because of the absence of excitation light. However, ultraviolet light is still necessary in common energy charging processes, which limits its reactivation in vivo because of its low penetration depth. In the present study, we introduce a type of hybrid nanocluster (UCPL-NC) composed of upconversion nanoparticles, β-NaYbF4:Tm@NaYF4, and persistent nanoparticles, Zn1.1Ga1.8Ge0.1O4:0.5%Cr, which can be activated by a 980 nm laser and exhibits an afterglow at 700 nm to realize near-infrared (NIR) to NIR UCPL bioimaging. The PL of the UCPL-NCs can be reactivated even when covered with a 10 mm pork. We demonstrate that these polyethylene glycol-modified phospholipid-functionalized UCPL-NCs can be reactivated in vivo and applied in the PL lymphatic imaging on small animals.