Impedimetric Bacterial Detection Using Random Antimicrobial Peptide Mixtures.
Tal Stern BauerRavit YakobiMattan HurevichShlomo YitzchaikZvi HayoukaPublished in: Sensors (Basel, Switzerland) (2023)
The biosensing of bacterial pathogens is of a high priority. Electrochemical biosensors are an important future tool for rapid bacteria detection. A monolayer of bacterial-binding peptides can serve as a recognition layer in such detection devices. Here, we explore the potential of random peptide mixtures (RPMs) composed of phenylalanine and lysine in random sequences and of controlled length, to form a monolayer that can be utilized for sensing. RPMs were found to assemble in a thin and diluted layer that attracts various bacteria. Faradaic electrochemical impedance spectroscopy was used with modified gold electrodes to measure the charge-transfer resistance (R CT ) caused due to the binding of bacteria to RPMs. Pseudomonas aeruginosa was found to cause the most prominent increase in R CT compared to other model bacteria. We show that the combination of highly accessible antimicrobial RPMs and electrochemical analysis can be used to generate a new promising line of bacterial biosensors.
Keyphrases
- label free
- ionic liquid
- pseudomonas aeruginosa
- loop mediated isothermal amplification
- dual energy
- computed tomography
- gold nanoparticles
- image quality
- staphylococcus aureus
- high resolution
- dna binding
- escherichia coli
- neural network
- real time pcr
- molecularly imprinted
- solid state
- biofilm formation
- transcription factor
- multidrug resistant
- current status
- antimicrobial resistance
- human health