An 'AND'-based ratiometric fluorescence probe for the sequential detection of biothiols and hypochlorous acid.

Hong ZhangLing-Ling LiLei ShiShang-Yong Chen Kun Li Xiao-Qi Yu

Published in: Chemical communications (Cambridge, England) (2022)

An 'AND'-based ratiometric fluorescence probe for the sequential detection of biothiols and hypochlorous acid was developed. FRET was observed only when RSHClO reacted with biothiols before reacting with hypochlorous acid, a phenomenon that has been confirmed in aqueous solutions and cells. This feature enables the probe to mimic biological processes and is particularly suitable for imaging oxidizing and reducing substances that cannot coexist.

Keyphrases

fluorescent probe
living cells
single molecule
induced apoptosis
loop mediated isothermal amplification
high resolution
machine learning
energy transfer
oxidative stress
real time pcr
mass spectrometry
cell death
photodynamic therapy
endoplasmic reticulum stress