Accelerated embryonic stem cell screening with a highly efficient genotyping pipeline.

Roger CaothienCharles YuLucinda TamRobert NewmanBrian NakaoTuija AlcantarNatasha BacarroJuan ReyesAnna PhamMerone Roose-Girma

Published in: Molecular biology reports (2022)

In sum, we describe a novel and effective implementation of ddPCR, multiMACS, and adenovirus recombinase into a streamlined screening workflow that significantly reduces timelines for gene targeting in mouse ES cells.

Keyphrases

highly efficient
stem cells
induced apoptosis
genome wide
cell cycle arrest
primary care
healthcare
high throughput
cancer therapy
copy number
oxidative stress
cell death
electronic health record
gene expression
signaling pathway
gene therapy