Frequent false-negative immunohistochemical staining with IDH1 (R132H)-specific H09 antibody on frozen section control slides: a potential pitfall in glioma diagnosis.

Akihiko YoshidaKaishi SatomiMakoto OhnoYuko MatsushitaMasamichi TakahashiYasuji MiyakitaNobuyoshi HiraokaYoshitaka NaritaKoichi Ichimura

Published in: Histopathology (2018)

We conclude that IDH1 (R132H)-specific (H09) immunohistochemistry often results in false-negative reactions on FS controls with focally increased background staining, and this application should be avoided in clinical practice.

Keyphrases

clinical practice
low grade
flow cytometry
wild type
risk assessment
high grade
human health