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Comparison of two LC-MS/MS methods for the quantification of 24,25-dihydroxyvitamin D3 in patients and external quality assurance samples.

Sieglinde ZelzerCaroline Le GoffStéphanie PeetersChiara CalapriceAndreas MeinitzerDietmar EnkoWalter GoesslerMarkus HerrmannCavalier Etienne
Published in: Clinical chemistry and laboratory medicine (2021)
This study is the first to assess the comparability of 24,25(OH)2D3 concentrations in a mixed cohort of hospitalized patients with two fully validated in-house LC-MS/MS methods. Despite different sample preparation, chromatographic separation and ionization, both methods showed high precision measurements of 24,25(OH)2D3. Furthermore, we demonstrate the improvement of accuracy and precision measurements of 24,25(OH)2D3 in serum samples and in the DEQAS program.
Keyphrases
  • end stage renal disease
  • ejection fraction
  • newly diagnosed
  • quality improvement
  • simultaneous determination
  • molecularly imprinted
  • tandem mass spectrometry