Three new compounds with their anti-glioma effects from the roots of Arnebia guttata Bunge.

Three new compounds, arneatas A-C ( 1 - 3 ), together with three known compounds ( 4 - 6 ) were isolated from the roots of Arnebia guttata Bunge. The structures were established on the basis of extensive spectroscopic data including NMR and HRESIMS. All the new compounds ( 1 - 3 ) were tested for their cytotoxic activity against two glioma cell lines (U118-MG and U373-MG) in vitro after treatment for 48 h. Compound 1 exhibited moderate cytotoxic activity against U118-MG and U373-MG glioma cell lines, with IC 50 values of 10.4 and 17.5 µM, respectively.