Postprandial Plasma Glucagon Kinetics in Type 2 Diabetes Mellitus: Comparison of Immunoassay and Mass Spectrometry.

Takehiro KatahiraAkio KanazawaMai ShinoharaMami KoshibuHideyoshi KagaTomoya MitaYuka TosakaKoji KomiyaTakeshi MiyatsukaFuki IkedaKosuke AzumaNaoko TakayanagiTakeshi OgiharaChie OhmuraAtsushi MiyachiEri MienoSatoko YamashitaHirotaka Watada

Published in: Journal of the Endocrine Society (2018)

Plasma glucagon levels during a meal test in patients with type 2 diabetes measured using ELISA were consistently higher than those measured using LC-HRMS. However, given that the changes in glucagon levels measured using ELISA before and after dipeptidyl peptidase-4 inhibitor therapy were similar to those based on LC-HRMS, this ELISA seems to be useful for evaluating the effect of the drug interventions on postprandial glucagon levels.

Keyphrases

mass spectrometry
liquid chromatography
high resolution mass spectrometry
blood glucose
simultaneous determination
physical activity
monoclonal antibody
type diabetes
stem cells
emergency department
blood pressure
bone marrow
adipose tissue
solid phase extraction
replacement therapy
label free