New Insights into the Structure of Kappa/Beta-Carrageenan: A Novel Potential Inhibitor of HIV-1.
Irina YermakStanislav AnastyukAnna KravchenkoWilliam HelbertValery GlazunovAndrey ShulginPavel V SpirinVladimir PrassolovPublished in: International journal of molecular sciences (2021)
New insights into the structure of the hybrid κ/β-carrageenan (κ/β-CRG) of the red alga Tichocarpus crinitus have been obtained. Carrageenan oligosaccharides were prepared through the chemical and enzymatic depolymerization of κ/β-CRG with κ-carrageenase and its the enzyme-resistant fraction. The composition and distribution of the repetition units of κ/β- CRG were investigated by using the negative ion tandem MALDI-TOFMS and ESIMS method, which made it possible to prove and characterize the hybrid structure of this polysaccharide. An analysis revealed the blockwise distribution of the long β-blocks along the polysaccharide chain, with the inclusion of κ/β, μ/ν-blocks and some ι-blocks. Furthermore, the desulfated κ/β-CRG was shown to contain of -G-D- repeating units up to 3.5 kDa. Previous studies have demonstrated that CRGs suppress the replication of several viruses. Here, we established that κ/β-CRG and its oligosaccharides significantly inhibit the transduction efficiency of replication-defective lentiviral particles pseudotyped with the envelope proteins of three different viruses. We found that the polysaccharide and its oligosaccharides strongly reduced the transduction efficiency of lentiviral particles pseudotyped with GP160-the envelope protein of the human immunodeficiency virus HIV-1-when added to T-lymphocyte Jurkat cells. The CRG oligosaccharides displayed significantly higher antiviral activity.
Keyphrases
- human immunodeficiency virus
- antiretroviral therapy
- hepatitis c virus
- hiv infected
- hiv positive
- hiv aids
- hiv testing
- induced apoptosis
- men who have sex with men
- mass spectrometry
- gene therapy
- cell cycle arrest
- immune response
- south africa
- hydrogen peroxide
- climate change
- single cell
- water soluble
- nuclear factor
- cell death
- toll like receptor
- protein protein
- risk assessment
- high resolution