Quantitative urinalysis using aggregation-induced emission bioprobes for monitoring chronic kidney disease.
Tong ChenNi XieLucia VigliantiYabin ZhouHui TanBen Zhong TangYouhong TangPublished in: Faraday discussions (2019)
Early detection and appropriate management of chronic kidney disease can reduce the progression of kidney failure and cardiovascular disease. The urine albumin to creatinine ratio (UACR) test is a standard urine test for identifying individuals at high risk of developing progressive kidney disease. In this study, IDATPE, a novel fluorescent probe with aggregation-induced emission (AIE) features, is successfully developed for creatinine detection and quantitation. An excellent correlation between fluorescent light intensity and creatinine concentration is achieved. In addition, BSPOTPE, a reported excellent AIE bioprobe for human serum albumin (HSA) quantitation, is used together with IDATPE in artificial urine for UACR testing. The mutual interference of HSA and creatinine when the bioprobes are used for quantitation is characterised, with promising results. Further improvements and potential applications in CKD quantitation are highlighted.
Keyphrases
- fluorescent probe
- chronic kidney disease
- living cells
- ms ms
- mass spectrometry
- liquid chromatography tandem mass spectrometry
- end stage renal disease
- uric acid
- liquid chromatography
- high performance liquid chromatography
- tandem mass spectrometry
- cardiovascular disease
- human serum albumin
- solid phase extraction
- multiple sclerosis
- metabolic syndrome
- single molecule
- simultaneous determination
- quantum dots
- peritoneal dialysis
- high resolution
- type diabetes
- high intensity
- risk assessment
- solid state
- coronary artery disease
- human health
- sensitive detection