Target-Dependent Compartmentalization of the Corelease of Glutamate and GABA from the Mossy Fibers.

The mossy fibers transiently corelease glutamate and GABA onto pyramidal cells of CA3. We here describe that they can also corelease these amino acids onto interneurons, in a target-dependent manner. Many interneurons in stratum lucidum and stratum radiatum receive both signals, while those in stratum lacunosum-moleculare exclusively receive a glutamatergic signal. It is noteworthy that glutamatergic LTD, which is known to exist on stratum lucidum interneurons, coexists in the same pathway with a presynaptic form of GABAergic LTP, while interneurons of stratum radiatum, despite receiving this dual signaling, do not display such plasticity. The GABAergic LTP is mimicked with PKA or PKC activation. We disclose compartmentalized corelease of glutamate and GABA and its differential plasticity from a single pathway onto different interneuron sets.